Bunnik Lab

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Handbook of Operations

Environmental Health & Safety

  Medium for Plasmodium falciparum

  Culturing 293T cells

  Culturing 3T3 cells

  Culturing THP-1 cells

  Memory B-cell isolation

  Transfection of 293T cells

  Thawing cell lines

  Human IgG ELISA

  Mycoplasma test
CHEMICALS - SAFETY DATA SHEETS

24. Sulfuric Acid (16%)

23. Bleach or hypochloride (8.25%)

22. Beta-MercaptoEthanol or BME or 2-MercaptoEthanol

21. DMSO or DiMethyl SulfOxide

20. DTT (1M)

19. Ampicillin sodium salt

18. EDTA solution

17. Ethanol 200 proof

16. Gentamicin solution

15. Giemsa stain solution

14. Halt protease inhibitor cocktail

13. Kanamycin sulfate powder | SOP

12. KAPA HiFi hotstart readymix

11. Methanol

10. Mycoalert plus mycoplasma detection kit

9. Penicillin / Streptomycin (Pen/Strep) liquid

8. Qiagen buffer N3

7. Qiagen buffer P2

6. Qiagen buffer PB

5. RNase away

4. SimplyBlue safe stain

3. Sodium Hydroxide (NaOH) solution (1M)

2. TMB

1. Trypan blue | SOP

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